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Country: United States
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we can get the dna sequence of the range of chromosome in human genome by using ucsc web tool.. extract amino acid position from excel file do*nloaded from gnomad export. we can simply use the following formulae to extract the number from string in excel file a comment on extract amino acid position from excel file do*nloaded from gnomad export create dictionary (optional) if you do not have the dict of reference fasta, you need to create select the original genome, original as*embly, new genome and new as*embly, say paste the data and click “submit” bu*ton as the rhs of the text area i want to do*nload the fastq files from basespace to the linux server directly without first do*nloading to local pc based on the project. i but, the python run do*nloader will do*nload files based on the run id that i want to do*nload based on the project. therefore, i modified from urllib2 import request, urlopen, urlerror import json def restrequest(rawrequest):  request = request(rawrequest) def do*nloadrestrequest(rawrequest,path):  dirname = projid + os.sep + os.path.dirname(path) except socket.error:  print 'got a socket error: retrying' projid = options.projid accesstoken = options.accesstoken #step 1: find the biosample id from project id first #as*ume fewer than 1000 samples in a project #step 3: get the do*nload filepath (hrefcontent) and filename (path) #normally two files per dataset in our case files are saving to the subdirectory with directory name is in one of the project, we need to get the genomic sequence of a specified region. in this case, i use the api by ensemble in my case, i need to get the human (hg19), so i can simply paste the following url on browser to get the result: if you have a list of range, you may write excel macro to help. for example, create a bu*ton and as*ociate with the following vba code. after filling the chromosome and range in colume a, b and c, and then click “submit” bu*ton. the sequence will be shown in the column d


Genome101 Main Page Content

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Title: GENOME 101 – Place to learn and enjoy Could be improved
Description: Place to learn and enjoy Could be improved
H1: GENOME 101Is it informative enough?
H2: Get the DNA sequence of the range of chromosome in human genome

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/about/:
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About – GENOME 101

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Hello! This is Mullin Yu. I am a novice in Bioinformatics and I would like to share the knowledge and information I found. I do hope this is also beneficial to all new joiners of this interesting and challenging field. Please drop me an email and comment if you have any suggestion. Enjoy!!

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/2018/12/06/get-the-dna-sequence-of-the-range-of-chromosome-in-human-genome/:
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Get the DNA sequence of the range of chromosome in human genome – GENOME 101

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We can get the DNA sequence of the range of chromosome in human genome by using ucsc web tool. http://genome.ucsc.edu/cgi-bin/das/hg19/dna?segment=chr8:126544471,126546471

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Get the DNA sequence of the range of chromosome in human genome

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genome101 – GENOME 101

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Read all of the posts by genome101 on GENOME 101

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Author: genome101

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Get the DNA sequence of the range of chromosome in human genome

/2018/12/05/extract-amino-acid-position-from-excel-file- [censorship] ed-from-gnomad-export/:
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Extract amino acid position from Excel file ed from gnomAD export – GENOME 101

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Description

We can simply use the following formulae to extract the number from string in Excel file =SUMPRODUCT(MID(0&E4, LARGE(INDEX(ISNUMBER(--MID(E4, ROW(INDIRECT(1:&LEN(E4))), 1)) * ROW(INDIRECT(1:&LEN(E4))), 0), ROW(INDIRECT(1:&LEN(E4))))+1, 1) * 10^ROW(INDIRECT(1:&LEN(E4)))/10) Note: Tested in Excel 2010

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Extract amino acid position from Excel file ed from gnomAD export

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/2018/12/04/how-to-liftover-vcf-file-by-batch/:
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How to liftOver VCF file by batch? – GENOME 101

Description

We can use the tool picard to liftOver VCF file by batch URL: http://broadinstitute.github.io/picard/command-line-overview.html#LiftoverVcf create dictionary (optional) if you do not have the dict of reference fasta, you need to create first a -jar /software/picard-tools-2.8.1/picard.jar CreateSequenceDictionary REFERENCE=/software/GATK_files/hg38/hg38.fa OUTPUT=/software/GATK_files/hg38/hg38.dict the chain file Go the http://hg .cse.ucsc.edu/ s.html#human and from liftOver hyperlink liftOver a -jar /software/picard-tools-2.8.1/picard.jar LiftoverVcf…

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How to liftOver VCF file by batch?

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