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Report of Laboratory Works Author: Sattf Fazeli Experimental Theory: MicroRNA replication using Real-time PCR One of the most popular techniques in the field of microRNA research. The basis of this technique is similar to the usual methods of Real-Time PCR, with the difference that small ranges of microRNAs have been proposed for this purpose. Adding a polyA tail to the desired miRNAs is one of the ways that the PARSGENOME MiR-Amp kit is also used to reproduce miRNAs. Extracting RNA is one of the essential steps in most research in the field of miRNA. There are different methods for extracting total RNA, but only some of them can maintain and extract small molecule RNAs. The following guidelines have been optimized and tested by our research team to extract total RNA containing miRNA. This instruction is applicable to extraction solutions based on guanidine isothiocyanate-phenol: chloroform. Extracting RNA is one of the essential steps in most research in the field of miRNA. There are different methods for extracting total RNA, but only some of them can maintain and extract small molecule RNAs. The following guidelines have been optimized and tested by our research team to extract total RNA containing miRNA. This is an instruction for extraction solutions with