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Most RecentMost CommentedLast ModifiedAlphabetical Show: 15101520253035404550 Filter Introduction Methyl green is used commonly with bright-field microscopes to stain cell nuclei by dyeing the chromatin of cells  with a green color. It is chemically related to cresyl violet. Materials 0.1M Sodium Acetate Buffer, pH4.2 Sodium acetate, trihydrate (MW 136.1) —— 1.36 gDistilled water ———————————– 100 mlMix to dissolve and adjust Avidin-Biotin blocking solution is used to reduce non-specific background that is commonly as*ociated with biotinylated antibodies. A SDS antigen retrieval solution can dramatically improve staining in paraformaldehyde-fixed, cryostat-sectioned tissue. A Tris Buffer antigen retrieval solution is a basic pH solution that is used to improve staining in paraffin embedded, formalin or A Tris Buffered Saline (TBS) antigen retrieval solution is used with paraffin embedded, formalin or paraformaldehyde-fixed tissues. Comments Off on Tris Buffered Saline (TBS) Antigen Retrieval Protocol A Tris-EDTA buffer antigen retrieval solution is used with paraffin embedded, formalin or paraformaldehyde-fixed tissues. EDTA buffer is an antigen-retrieval solution used to improve staining for low abundance epitopes. Citrate EDTA buffer antigen retrieval solution is used with paraffin embedded, formalin or paraformaldehyde-fixed tissues. Citrate buffer antigen retrieval is a solution used at elevated temperatures to improve immunohistochemical staining in fixed tissues.